Pathogen Detector (Contest Entry)

8d ago (edited) in Entries

Portable CRISPR Device for Real-Time Microbial Detection in Water Sources

Any feedback or comments on my project are greatly appreciated!

Microbial contamination of drinking water poses significant risks to public health, particularly in areas with limited access to laboratory-based water testing methods (Ashbolt, 2015). Traditional techniques, such as microbial culturing and polymerase chain reaction (PCR), are effective but impractical for real-time, field-based applications due to their cost, time requirements, and reliance on specialized infrastructure (Yu et al., 2005). This study proposes a portable CRISPR-based device for rapid, real-time detection of microbial pathogens in water. The device integrates modular components for filtration, cell lysis, genetic material extraction, and CRISPR-Cas12/Cas13-based detection, with fluorescence or colorimetric outputs to provide accurate and timely results (Tian et al., 2024). Methodologies for pre-filtration, lysis chamber design, and CRISPR reaction setup are outlined. Future advances include AI-enhanced signal analysis and multiplexed pathogen detection.

Why Does This Matter?

Current water testing techniques often fail to meet the needs of vulnerable populations due to their complexity, cost, and dependence on laboratory infrastructure. My device bridges this gap, providing a simple and effective solution to detect waterborne pathogens in real time. With potential applications in households, disaster zones, and industrial water monitoring, this device could significantly improve global health outcomes. The funding from this contest would enable the acquisition of necessary materials for further testing and optimization. If you believe in the power of innovation to solve the water crisis and save lives, consider supporting this project!

https://docs.google.com/document/d/1mEhQnTgx47RrZWAklg6OH4O4qSzfmooUIxwN3uVEqFA/edit?usp=sharing

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